ABSTRACT Designing coordination compounds by ligand tailored with therapeutic abilities has become a fascinating field.From this perspective, a cobalt(II) coordination compound of 1,10 Phenanthroline ,2,2’ Bipyridine and acetamide were synthesized by the reaction between cobalt(II) salt and ligands in molar ratio (1:1:1:1),where M=Co and the ligands are1,10 Phenantroline, 2,2’Bipyridine and acetamide. The compound was characterized by using infra red, electronic spectra, halide test, solubility test, melting point, conductivity and metal content using ICP OES. Octahedral geometry with formula [Co(Phen)(Bpy)(Act)(H2O)]Cl2 is proposed. The spectroscopic data suggests that the ligands are coordinated with the metal ion via nitrogen donor centers. The complex was tested for antibacterial activity against two Gram positive Staphylococcus aureus and Streptococcus pyogenes and two Gram negative Escherichia coli and Klebsiella pneumonia bacteria.The antibacterial activity of the complex is found to be active against all the tested bacteria and demonstrated good inhibiting activity even in Gram negative bacteria (Escherichia coli, Klebsiella pneumonia) the complex showed lower activity than the reference antibiotic drug (Gentamycin).