Milk is an important source of nutrients to human and animals. However, due to its high water activity and nutritional value, it serves as an excellent medium for growth of many kinds of microorganisms under suitable conditions. The present study deals with comparative analysis of the microbiological quality and safety of raw cow milk samples collect from Andassa Livestock Research Center (ALRC) and Bahir Dar Dairy Cooperatives (BDDC). A total of 60 samples (30 from each site) of raw cows’ milk were collected. Two hundred ml of raw milk sample were taken aseptically from mixing container for quantification of Aerobic Mesophilic Bacteria(AMB), Staphylococcus aureus, Total Coliform (TC), Fecal Coliform (FC) andYeasts and Moulds. In addition, isolation of Brucella and Salmonella was conducted in all samples. The mean values of thefive quality parametersin the milk samples of both sites were: AMBC (ALRC,7.47±0.06;BDDC,6.97±0.4logcfu/ml), S.aureus(ALRC,5.30±0.13log10cfu/ml;BDDC,4.75±0.46log10cfu/m),TC(ALRC,2.94±0.23logMPN/ml;BDDC,2.43±0.53logMPN/ml),FC(ALRC,1.80±0.69logMPN/ml; BDDC,1.18±0.40logMPN/ml) and Yeast and Mould (ALRC, 6.41±0.084 log10cfu/ml; BDDC, 5.13±0.19log10cfu/ml).From the 60 samples, Brucella was detected in 19(31.6%)samples (ALRC, 8(26.6%); BDDC, 11 (36.6%)) and Salmonella was detected in 10(16.6%) samples (ALRC 6 (20%); BDDC, 4 (13.3%)).The results shows that the microbial quality of raw milk obtained from ALRC was significantly higher than (P = 0.00) the sample obtained from BDDC. All the parameters did not satisfy the WHO standards.A source of water for washing milking utensils (0.002), practice of washing hands (0.021), the frequency of barn cleaning (0.012) and frequency of washing milking utensils and collectingtanks(0.033) were significantly associated with the microbial count of the milk. Such high microbial load and detection of pathogenic bacteria clearly reflect the poor hygienic practices during time of milking and subsequent handling. Therefore, proper hygienic practice will be very important for dairy farmers in the study sites so as to improve safe dairy production. Key Words:Andassa Livestock Research Center, Bahir Dar DairyCooperatives, Microbial quality, Raw Milk.