Abstract:
111~ antioxidant and anttbacteria! activity' of Rumrx abyssinicus IR.4j Zingiber ojficlr111/e (ZOJ and
Curcvma longo (CLj samp!« exrrnc:t.~ were studied. Phvtochemtcal screening test» were. first, done using'
dif!?ent ct1njimrutory tests prior 111 qualntiuniv« determtnatious. 1kcordi11gll', oll of the samples ~hm1'•'d
pclli11Jv., 1e.11s. with d!f{erent ime11.1iry, .fiir the presence Q,f phenolics, jlavonoids, glyccsid~.• turd tannin»:
bw JIQ/ Jar olkotoids. Qmmtilaliv<' determinottons were then conducted, and if was jo1111c1 that RA was
dominant /11 al! thre« determinattons tile total phenolic conteut by the Prussian blue method, total
flavonoid co111enl by th« a/im1inul!!. colorimetric assay, and antioxldant uclivi(• by tire rc·ducing power
as.wy und th« cyclu: voltummetry technique. 8(1SC'd 0111hls. rhe l(){lli phenolic- 'w1te111 of RA. ZO and Cl,
as calculated in terms of m,I!. galik udd equivalent per g <!/ dry weigh I of sample, wa.1 3I9>15. 997.
81. 108:./.815. 201.106~45. lfi.l. respentvety. Ukewise, the 1ofaljluHmo1d cotuen: in 1'1.< s11111e order, '"
c:alculated in terms cf mg q'!f/'·c:tin per g of dry ll'eight of sample, was 113 ZS2'1./.71J2. ./2.355+.J.67/l,
17.8]Q~l!.4fl5. respectively. Whi!« R.4 ossumed if) superiority in th« antioxktant acli•iry tu caicuiated in
terms of mg ascorbic odd equivuien/, 100. there l•'as 011/11 a ,,/ighl differenc« i11 those 11/' ZO and Cl.
Accordingly, th« antioxidant activities wt1re ./5.6.U ... 2.026, 10.IJ(}(j,.IJ../96, 9.7JiJ0.578jor RA, t:O and
CL respecyivel»: 0!1 the other bond. l101h oxidatlou and reduction peaks weri· observed i11 the cycli«
voltammetry onalysis of 1'1e her/is, shm1·inf1 their reduc<'d antioxtdont actil'/1y relative IO thei» rolol
phenolic andfltrvonotd contents. Wir!t regard 1<> the antibaaerta! acuvuv determination. cif1<m11I extract
of R.~ shdwed stronger 1:(fec1 l!UJ11 us chloroform correspondent tmd KW effidet// ogains: all the tested
microrganistns relative to ZO and CL. Ill this dtnermination. 1'1• inhtbtton Wile> (in r71111) of ethano!
extract of RA werf! found to he 10. JJJ.{I. 58. 11.67+0. 58, 19.17'1.0.}9. /fi.17 t-0.19, and :!l.67+'.J.58 against
Slaph):/(ICOG~·"J aurous, :lfRSA, Streptococcus pneumonia; t7scltericin coli, and Sh/gel/a .P~xe11eri
bacteria, re:pec1i,,dy. Tire stronger acuvity f!f RA /11 both tests showed Thal tbe troditiono! use ofrhe herb