This study was conducted to analyze the major phytochemicals,to evaluate the antioxidant capacities by using, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities and ferric reducing antioxidant potential (FRAP)as well as to determine the total flavonoid content and antimicrobial activities ofleaf extracts of Otostegia integrifolia with different solvents.The percentage yields of methanol, ethyl acetate and petroleum ether extracts were 21.84%, 13.28% and 2.07% respectively.Different bioactive compounds were present in each extracts.The antioxidant potential increased with increasing concentration of the sample.The result of total flavonoid content estimation in methanol and ethyl acetate crude extract was found to be 416.5 ±0.288mgQE/100g and 248.9 + 0.872mgQE/ 100g dry sample respectively. The DPPH scavenging capacity of methanol and ethyl acetate extracts were 82.91+ 0.365 and 32.68 + 1.545 respectively and the FRAP values of methanol and ethyl acetate extracts were 286.146 + 0.889 and 219.496 + 0.566mgAAE/100g respectively.The methanol leaf extract showed high antioxidant activity than leaf extract of ethyl acetate.Growth inhibition tests against bacteriashowed a good result at a higher concentration in methanol and ethyl acetate extracts, butinhibition zone were not observed in the petroleum ether stem extracts in all bacterial strain. Key words: Traditional medicine, Otostegia integrifolia, antioxidant activity, antimicrobial activity, total flavonoid content, minimum zone of inhibition