Abstract:
Pepper (Capsicum annuum)is a staple spice food for more than half of the population ofthe world. In Ethiopia, for example, it is the mostcommon and cultivated food species. In this work Flame Atomic Absorption techniquewas used to determine the concentrations of essential metals (Ca, Mg, Cr, Cu, Fe, Mn and Zn) and non-essential metals (Cd and Pb) in pepper and soil samples. The pepper and Soil samples were collected from South Gondar, FogeraWoreda, Khar Abo Kebele. The optimized wet digestion procedure was evaluated using standard addition (spiking) method and an acceptable percentage recovery was found to be from 90-105 and90-99 for the metals in pepper and soil samples respectively. 1 g of oven dried pepper sample was digested using 3:2:1 ratio of 12 mL HNO3, HClO4, and H2O2 at 250 ° C for 2:30 hr and 1 g dried soil sample was digested with reagent mixture ofHCl, HNO3 and H2O2(with 3:1:1 ratio respectively) at 250 °C for 3:00 hr. Among the studied metals, Fe was found in maximum amount and Ca was also the second abundant metal in the study area.In the present study it was found out that all types of pepper contain considerable concentration of trace metals except Cd and Pb.The concentrations of metals in this pepper were comparable to values reported in literature from different parts of the world. The levels of all the studied metals in the samples were below the WHO allowed limit.
Keywords:Pepper(Capsicum annuum), essential and non-essential metal, FAAS (Flame Atomic absorption spectrophotometer), trace metals