Phytochemical, Antioxidant and Antimicrobial Studies of Ethanolic and Methanolic Extracts of Aloe elegans Leaves gel

Halefom, Nigus

dc.contributor.author	Halefom, Nigus
dc.date.accessioned	2017-10-11T04:55:06Z
dc.date.available	2017-10-11T04:55:06Z
dc.date.issued	2017-10-11
dc.identifier.uri	http://hdl.handle.net/123456789/7916
dc.description.abstract	The present study aimed at investigating the phytochemical, antioxidant and antibacterial activities of the ethanolic and methanoli extracts of Aloe elegans leaves gel. The phytochemical screening revealed the presence of phenols, flavonoids, anthraquinones, tannins, sapponins and terpenoids in both extracts. The antioxidant activities of both extracts were assayed using ferric reducing antioxidant power (FRAP), conjugated diene (CD) and peroxide value (PV) methods using sunflower oil (SFO) as the oxidizable substrate. The antioxidant activities of the extracts were compared with those of the familiar compound vitamin E. The antibacterial activities of the extracts were likewise evaluated using agar well diffusion method using the known antibiotic Gentamicin as a reference. The value for methanol and ethanol extracts of Aloe elegans leaves gel and the standard ascorbic acid in the ferric reducing power assay at 20 μg/mL were found to be 65.32%, 62.89%, 59.4% respectively. The peroxide value at the end of the storage period of 12 days at room temperature for the control Sunflower oil (SFO) was found to be 45 mmol/L while the value for the substrate containing methanol, ethanol extract and vitamin E were found to be 36, 38, 43 mmol/L respectively. The value of the absorption coefficients of conjugated dienes formed as a result of oxidations of the methanol and ethanol extracts and vitamin E were found to be 3.006, 3.387, 4.306 g -1 cm -1 mL respectively. In all the antioxidant assays used in this work, the methanolic extract was found to inhibit oxidation of sunflower oil to a better extent than its ethanolic counterpart. Relative to vitamin E, both the methanolic and ethanolic extracts exhibited better antioxidant efficacy. In the antibacterial assay, both the extracts showed potent bactericidal effects against Escherichia coli and Staphyloccus aureus, though to a lesser extent than the standard antibiotic Gentamicin.	en_US
dc.subject	chemistry	en_US
dc.title	Phytochemical, Antioxidant and Antimicrobial Studies of Ethanolic and Methanolic Extracts of Aloe elegans Leaves gel	en_US
dc.type	Thesis	en_US