This study was conducted from May to December2016 and the aim was to investigate the spatio-temporal distribution of Labeobarbus species and the effect of gillnet selectivity. Fish were collected monthly at four sampling sites (Bahir Dar, Kunzla, Gumara and Gorgora) using a gillnets of 6, 8, 10, 12, and 14 cm stretched mesh size. . Descriptive statistics was used to present the data. Chi-Square Test was used to compare the spatial variations of species between areas, the temporal variations between seasons. Their distribution, relative abundance and diversity was determined by the IRI and Shannon diversity index (H') value respectively. A total of 2662 Labeobarbus specimens were collected within eight sampling months at all sampling sites. Labeobarbus intermedius was the most dominant specie, containing 38.8 % of catch followed by L. tsanensis, L. platydorsus, L. brevicephalus within a catch composition of, 18.96%, 13.1%, 10.5%, respectively. Labeobarbus dainellii and L. gorguari were rarely collected. Labeobarbus species of Lake Tana showed significant variation in catches at four sampling sites (p<0.01) and also these species showed significant variation in dry and rainy seasons (p<0.01). L. intermedius, L. tsanensis, L. brevicephalus, L. nedgia, L. gorgorensis, L. truttiformis were significantly more abundant in