PHYTOCHEMICAL AND PROXIMATE ANALYSIS ON THE LEAF EXTRACTS OF  Corchorus olitorius (KUDRA)

ZIMIE, AMIBAW

dc.contributor.author	ZIMIE, AMIBAW
dc.date.accessioned	2024-12-17T07:51:39Z
dc.date.available	2024-12-17T07:51:39Z
dc.date.issued	2024-06
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/16397
dc.description.abstract	In ancient times people used plants for treatment of different types of disease. Phytochemicals are compounds that occur naturally in plants. Corchorus olitorius (family; Tiliaceae) locally known as “Kudra” is used as a traditional medicine and food in the warmest area of Ethiopia specifically in Quara woreda (West Gondar Zone). The plant is found naturally in the field. This plant is famous in Asia and other African countries. This study aimed to determine the total phenolic content, total flavonoid content, antioxidant activity, and antibacterial activity of leaf extracts by different solvent and also to determine proximate compositions. The leaf of Corchorus olitorius was collected from Gelego local market, which is the capital city of Quara woreda in the west Gondar administrative zones. Methods of quantitative determinations of the leaf extracts are Folin-Ciocalteu method, aluminum chloride assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and disc diffusion standard methods were used to determine the total phenolic content, total flavonoid content, antioxidant activity, and antibacterial test, respectively. The Oven dry method, Muffle furnace, Kjeldahl method, Soxhlet method, and acid and base digestion standard methods were used to determine moisture, total ash, crude protein, crude fat, and the crude fiber contents of leaf, respectively, for this study. The finding for the current study of the plant total phenolic content and total flavonoid contents in different solvent extractions varied from 3.62 ± 0.35 – 33.62 ± 0.25 mgGAE/g and 7.28 ± 0.05 – 30.76 ± 0.01 mgQE/g dry extract, respectively. The percent inhibition for ascorbic acid, ethanol, acetone, and petroleum ether extract varied from 46.70 - 80.38, 40.08 - 65.46, 31.34 - 60.55 and 7.24 - 18.76, respectively. The percentage reduction power assays for ascorbic acid, ethanol, acetone, and petroleum ether extracts varied from 54.18 – 78.92, 45.2 -76.82, 31.84 -75.36 and 17.46 -50.36, respectively. The moisture, crude protein, crude fat, crude fiber, total ash, total carbohydrate, and the total energy values were 10.53 ± 1.23, 17.24 ± 0.49, 2.83 ± 0.49, 14.70 ± 0.67, 11.50 ± 1.08, 43.2 ± 0.79 and 267.23 ± 3.18, respectively. The extracts of high polar solvents showed higher phenolic and flavonoid contents as well as antioxidant activity. The IC50 value of petroleum extract was 0.24021mg/mL. This result indicates, petroleum ether extract was lowest antioxidant activity as compared to ethanol and acetone extract. Corchorus olitorius leaves are measureable in moisture, total ash, crude protein, crude fat, crude fiber, and total carbohydrate. Both extracts had good antibacterial activity in gram negative and gram positive bacteria. Corchorus olitorius was found to be a good source of the six essential nutrients.	en_US
dc.language.iso	en_US	en_US
dc.subject	Chemistry	en_US
dc.title	PHYTOCHEMICAL AND PROXIMATE ANALYSIS ON THE LEAF EXTRACTS OF Corchorus olitorius (KUDRA)	en_US
dc.type	Thesis	en_US