Green Synthesis and Characterization of Silver Nano Particles Using Milletia Ferruginea (Birbira) Leaf  Extract for Antibacterial Activities

ESHETIE, ABEJE

dc.contributor.author	ESHETIE, ABEJE
dc.date.accessioned	2024-12-17T07:17:18Z
dc.date.available	2024-12-17T07:17:18Z
dc.date.issued	2024-12
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/16396
dc.description.abstract	Due to its benefits over physical and chemical techniques of synthesis, researchers have recently shown a great deal of interest in green synthesis of silver nanoparticles for a variety of applications. As a reducing and capping agent, M.ferruginea (Birbira) leaf aqueous extract was utilized in the current investigation to successfully create silver nanoparticles. The impacts of several reaction parameters, including incubation duration and extract-to-AgNO3 ratio, were optimized. The green synthesized silver nanoparticles were preliminarily confirmed by the visual color change. Furthermore, UV-visible, FT-IR, XRD and SEM methods were used to characterize the produced AgNPs. The surface Plasmon resonance (SPR) characteristic peak of silver nanoparticles was validated by the development of a prominent UV-Vis spectra peak at 419 nm. The presence of many Phytochemicals that reduce silver ions and are employed as capping agents for produced AgNPs was verified by the FT-IR spectroscopy study. The face centered cubic (FCC) structure of AgNPs, which have an average particle size of 8.960707 nm, was validated by X-ray diffraction (XRD) patterns. The development of uniformly dispersed spherical silver was validated by SEM examination. The antibacterial activity of synthesized AgNPs and plant extract was investigated against two gram-negative (E. coli and K. pneumonia) and two gram-positive (S. aureus and S. pyogenes) bacterial species by the disc diffusion method. Even at diluted concentrations, the data showed that AgNPs exhibited significant antibacterial action against all tested bacterial species. The green produced AgNPs had the highest ZOI at 100µg/ml (14.6±0.57mm, 14.3±0.57mm, 14.0±0,0mm, 14.6±0.57mm,) for S.pyrogens, S.aureus, K.pnemonia and E.coli bacteria respectively. The maximal ZOI of ciprofloxacin on S. pyogenes, S.aureus, K.pnemonia, and E.coli bacteria was 23±0.57 22±0.57, 21±0.0, 22±0.57. According to the current work, the green synthesized AgNPs made with Birbira leaf extract demonstrated strong antibacterial efficacy	en_US
dc.language.iso	en_US	en_US
dc.subject	Chemistry	en_US
dc.title	Green Synthesis and Characterization of Silver Nano Particles Using Milletia Ferruginea (Birbira) Leaf Extract for Antibacterial Activities	en_US
dc.type	Thesis	en_US