Phytochemical Screening, Determination of Antioxidant and Antibacterial Activities of the Stem Bark Extracts of  Azadirachta Indica (Neem) S

Asnakew, Dires

dc.contributor.author	Asnakew, Dires
dc.date.accessioned	2024-11-10T07:14:15Z
dc.date.available	2024-11-10T07:14:15Z
dc.date.issued	2024-10
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/16130
dc.description.abstract	The stem bark of Azadirachta indica, one of the most widely distributed plants in tropical and sub-tropical countries, is used in traditional medicine for treatment of several diseases. This study was conducted to analyze the major phytochemicals, to evaluate the antioxidant capacities using, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities as well as to determine the total phenolics, and the antibacterial activities of extracts of the stem bark of A.indica. The stem bark of this plant was extracted by successive extraction with petroleum ether, ethyl acetate and methanol solvents. The percentage yields of methanol, ethyl acetate and petroleum ether extracts were 4.48%, 2.75% and1.98% respectively. Qualitative phytochemical analysis of the extracts indicated the presence of, alkaloids, flavonoids, terpenoids, and phenols, with different concentration. The result of total phenolics content estimation in petroleum ether, ethyl acetate and methanol crude extract was 17.87, 32.14 and 55 mgGAE/g dry samples respectively. The DPPH radical scavenging assay result showed that methanol stem bark extract has high antioxidant activity than ethyl acetate and petroleum ether extracts. IC 50 values were found to be 34.96 μg/mL, 50.45 μg/mL and 68.56 μg/mL in methanol, ethyl acetate and petroleum ether stem bark extracts respectively for their antioxidant activity by DPPH assay; the lower IC 50 value which showed that the radical scavenge activity to be effective in this solvent extract. The antioxidant potential of the extracts increased with increasing concentration of the sample. Growth inhibition tests against gram positive (S. aureus and S. pyrogens) and gram negative (E. coli and K. pneumonia) bacteria showed a good result at a higher concentration in methanol and ethyl acetate extracts.	en_US
dc.language.iso	en_US	en_US
dc.subject	Chemistry	en_US
dc.title	Phytochemical Screening, Determination of Antioxidant and Antibacterial Activities of the Stem Bark Extracts of Azadirachta Indica (Neem) S	en_US
dc.type	Thesis	en_US