Phytochemical Screening, Antioxidant, Antibacterial Activities of The Leaf Extracts of Clematis Hirsuta (Buni Yeazo Hareg) and Green  Synthesis and Characterization of Cuo Nano Particles and its Essential Oil

Agegnehu, Demisew

dc.contributor.author	Agegnehu, Demisew
dc.date.accessioned	2024-11-10T07:09:27Z
dc.date.available	2024-11-10T07:09:27Z
dc.date.issued	2024-10
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/16129
dc.description.abstract	Traditionally in Ethiopia, Clematis Hirsuta leaves have been utilized to prevent diarrhea, tooth infection, and gonorrhea. This particular study aimed to examine the phytochemical composition, as well as the antimicrobial and antioxidant activities of the crude extract (CE), CuONPs, and essential oil (EO) of C. Hirsuta leaves. The synthesis of copper oxide (CuO) nanoparticles from CuSO 4 through the wet chemical precipitation method was characterized using X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM). Both CE, and EO were confirmed to contain phenolics, flavonoids, alkaloids, saponins, steroids, glycosides, terpenoids, and tannins through the phytochemical screening test. The highest amount of TFC 103.5 mg GLA/g was observed in the CE, whereas the lowest value was recorded for EO extracts. The antioxidant activity of each CE, CuONPs and EO was assessed using the DPPH free radical scavenging assay, and it demonstrated the highest antioxidant activity, surpassing that of standard ascorbic acid, which was less than 80%. EO exhibited the highest %inhibition at 80 ppm concentration, which was 94%. The GCMS results indicated that the EO of C. Hirsuta leaves possesses various chemical constituents, such as Pyrimidinone and 1-Hexadecanol, which may be accountable for pharmacological and antioxidant activities. Although the commercial drug demonstrated a higher inhibition zone than the extract, the obtained EO displayed superior antibacterial activity, followed by CuONPs, and CF extract, respectively. In conclusion, these findings suggest that C. Hirsuta leaves are a promising source of natural antioxidant capacity, which could have significant importance as a therapeutic agent in preventing or slowing down the progress of aging-associated oxidative stress related to degenerative diseases, such as diabetes, cancer, and cardiovascular diseases caused by a lack of antioxidant capacity.	en_US
dc.language.iso	en_US	en_US
dc.subject	Chemistry	en_US
dc.title	Phytochemical Screening, Antioxidant, Antibacterial Activities of The Leaf Extracts of Clematis Hirsuta (Buni Yeazo Hareg) and Green Synthesis and Characterization of Cuo Nano Particles and its Essential Oil	en_US
dc.type	Thesis	en_US