Phytochemical Analysis, Antioxidant and Antibacterial Activities of Ginger (Zingiber Officinale)

Deguale, Aynetu

dc.contributor.author	Deguale, Aynetu
dc.date.accessioned	2024-11-10T06:50:42Z
dc.date.available	2024-11-10T06:50:42Z
dc.date.issued	2024-10
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/16125
dc.description.abstract	Ginger (Zingiber officinale) is one of the most versatile medicinal plants that have been a variety of biological activities due to the presence of large number of bioactive compounds. The present study is conducted to evaluate phytochemical test, total phenolic content, antioxidant efficiency and antibacterial activities using methanol, chloroform and n-hexane extracts. Phytochemical screening of the crude extracts in methanol revealed the presence of alkaloids, phenolic compounds, saponins, flavonoids, terpenoids, sterols and glycosides. Chloroform extract of Zingiber officinale also contains alkaloids, phenolic, flavonoids, terpinoids, sterols and glycosides. The n-hexane extract of Zingiber officinale is the least phytochemical components which contains alkaloids, phenols, sterols and glycosides. The total phenolic content of methanol, chloroform and n-hexane was 88.57 mgGAE/g, 65.86 mgGAE/g and 62.08 mgGAE/g dry samples respectively. The antioxidant activities of each crude extract was screened using 2.2-diphenyl-1- picryl-hydrazyl (DPPH) assays. IC50 values of Zingiber officinale were found to be 52.439 ppm , 87.488ppm and 134.816 ppm in methanol, chloroform and n-hexane respectively for their antioxidant activity by DPPH assay; the lower IC50 value which showed the radical scavenge activity to be effective in this solvent extract. In the same condition methanol, chloroform and nhexane extract showed high antioxidant activities. The antibacterial activity of zingiber officinale extract was performed against four bacterials species S. aureus, E coli, S. pyrogens, K. pneumonia, at concentrations of 500 mg/mL, 250 mg/mL, 125 mg/mL and 62.5 mg/mL and 31.25 mg/ml. The result revealed that the methanol and chloroform extracts showed a better activity against all disease causing pathogens bacterial while, at higher concentration n-hexane extracts were active against microorganisms.	en_US
dc.language.iso	en_US	en_US
dc.subject	Chemistry	en_US
dc.title	Phytochemical Analysis, Antioxidant and Antibacterial Activities of Ginger (Zingiber Officinale)	en_US
dc.type	Thesis	en_US