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Bacteriological and Physicochemical Quality and Antibiotic Resistance Profile of Bacteria Isolates from Drinking Water in Bahir Dar Zuriya Rural Kebeles, West Gojjam, Ethiopia

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dc.contributor.author Wessen, Zinash
dc.date.accessioned 2024-03-21T08:15:26Z
dc.date.available 2024-03-21T08:15:26Z
dc.date.issued 2023-06
dc.identifier.uri http://ir.bdu.edu.et/handle/123456789/15702
dc.description.abstract Drinking water must be free from pathogenic micro-organisms, chemical, physical contaminants and other pollutants that constitute a danger to a person’s health. The objective of this study is to assess the bacteriological and physicochemical quality of drinking water in Bahir Dar Zuryia Rural District. A cross-sectional study was conducted from source of water and household container in Bahir Dar Zuryia Rural Kebele community from April 2022 to august 2022. Sixty water samples were collected from hand dug wells, river, hand pump and house hold containers from Bahir Dar Zuryia Rural Kebele and analyzed for total coliforms and fecal coliforms using the MPN method and the isolation of pathogens using selective medium for each pathogen. Antibacterial susceptibility profiling of the isolated bacteria was conducted on Mueller Hinton Agar, using a common Kirby-Bauer disk diffusion technique. The physicochemical parameters namely Temperature was measured by thermometer on site and pH was measured using a portable digital pH meter, Conductivity and Total Dissolved Solid (TDS) were measured by portable conductivity and TDS meter and Turbidity of the samples was determined using a turbidity meter in the laboratory. From a total of 60 samples, 91.6 % (55/60) and 40 % (24/60) of the drinking water samples were tested positive for Total Coliforms (TC) and Fecal Coliform respectively. From total pathogens, 16 (37.2%), 15 (34.8%) and 12 (27.9%) were positive for Pseudomonas spp. Salmonella spp. and Shigella spp. were found respectively. Most of isolated pathogen bacteria 97.6%% were resists at least one antibiotic and 62.8 % (27/43) bacteria were resist two and more than two antibiotics. The mean value of temperature, pH, conductivity, total dissolved solid and turbidity in all water samples were recorded 19.6 0 c, 6.76, 327µs/cm, 198.5 mg/l and 8.81 NTU, respectively. Among physicochemical parameters, pH, Conductivity and total dissolved solid mostly in line with WHO standards but temperature and turbidity water sample were not fulfilling WHO and National Standard Guide line. In this study, there is high level of total and faecal contamination of drinking water in Bahir Dar Zuriya Rural Kebele. High risk scores found in the drinking water suggest poor source and household container protection, poor sanitation conditions and practices en_US
dc.language.iso en_US en_US
dc.subject Biology en_US
dc.title Bacteriological and Physicochemical Quality and Antibiotic Resistance Profile of Bacteria Isolates from Drinking Water in Bahir Dar Zuriya Rural Kebeles, West Gojjam, Ethiopia en_US
dc.type Thesis en_US


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