Proximate Analysis, and Determination of Mineral Composition, Phytochemical Contents, Antioxidant and Antibacterial Activities of Methanol Extracts of Portulaca Qurdifirda L

Mengistu, Nigatu

dc.contributor.author	Mengistu, Nigatu
dc.date.accessioned	2023-07-13T08:42:39Z
dc.date.available	2023-07-13T08:42:39Z
dc.date.issued	2023-06
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/15506
dc.description.abstract	The use of medicinal plants to treat diseases is as old as mankind. Portulacea quadrifida (family:portulacea) commonly known as “chicken weed”, a traditional medicinal plant has been semi cultivated and used as traditional medicine and nutrients in Benshangul Gumuze Ethiopia. The plant also naturally found in lawns, and fields and in many regions of Ethiopia and Africa. The purpose of this study was to determind the proximate, mineral, anti-nutrients composition of the aerial part and to investigate various phytochemicals (qualitative), evaluate the TPC, TFC, antioxidant activity and antibacterial activity of the plant stem and leaf extracts with methanol solvents and to recommend its nutritional benefit by comparing with commonly consumed vegetables. Weight difference, Kjeldahl method, dry Soxhlet extraction, acid and base digestion, 100% - %(ash +moisture +protein + fiber, Folin Ciocalteu, aluminum chloride assay, DPPH scavenging, disk diffusion, standard methods were used to determined ash and moisture, crude protein, crude fat, crude fiber, carbohydrate, TPC, TFC, antioxidant, antibacterial, and qualitative phytochemical test respectively in this study. Finding of this study showed that the plant contained moisture (6.56±0.0092%) ashes (7.13 ± 0.14%), crude protein (17.02 ± 0.46%), crude fat (6.33 ± 0.039%), crude fiber (14.296 ± 0.487%) and total carbohydrate (46.977%). The plant has high energy values [312.95 kcal/100 g dry weight (DW)]. Mineral ranges (mg/100 g DW) were: K (19.80), Ca (7.97), Fe (7.77), Mg (21.133), P (11.8), and Zn (13.385). The study also showed that the amount of ant-nutritional factors such as Tannin, Phytate, and Oxalate were found to be lower than the RDA and the amount in commonly consumed vegetables. Comparing the mineral contents of P.quadrifida with common vegetables and RDA, the results indicated that the nutritive value of P quadrifida L. aerial part falls under the range of nutritive value of commonly consumed vegetables. Therefore P.quadrifida could be a good supplement for some nutrients such as protein, carbohydrates, Energy, Fe, Mg, Zn, and Na. Both extracts showed noticeable amount of TPC, TFC, antioxidant and antibacterial activities. TPC of the stem and leaf extracts were found to be 19.95 ± 0.0529 and 26.5256 ± 0.0529 mg GAE/g respectively. TFC of stem and leaf extracts varied from 2.553 ± 0.0772mgQE/g to 3.634 ± 0.0571 mg QE/g (methanol) extract respectively. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH), (%) radical scavenging activity was varied between 24.72-69.72% for stem and 32.85-75.85% for leaf methanolic extract based on the concentration of the sample	en_US
dc.language.iso	en_US	en_US
dc.subject	Chemistry	en_US
dc.title	Proximate Analysis, and Determination of Mineral Composition, Phytochemical Contents, Antioxidant and Antibacterial Activities of Methanol Extracts of Portulaca Qurdifirda L	en_US
dc.type	Thesis	en_US