Phytochemical Investigation, In Vitro Anti-Bacterial and Anti-Oxidant Activity and Gc-Ms Profile Of Essential Oils of Stem Bark Extract of Ficus Sycomorus L. (Bamba)

Fikadu Girma

dc.contributor.author	Fikadu Girma
dc.date.accessioned	2023-07-05T08:39:08Z
dc.date.available	2023-07-05T08:39:08Z
dc.date.issued	2023-07
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/15459
dc.description.abstract	Introduction: Ficus sycomorus (F. sycomorus), also known as fig-mulberry belonging to the family of Moraceae. It is locally named as “Bamba‖ in Amharic. The plant contains different groups of biologically active compounds which are responsible for the biological activity. It has several medicinal and culinary uses. Objectives: This study is aimed at investigating phytochemicals, evaluating their in vitro antibacterial and antioxidant activities, and analyzing the GC-MS profile of essential oils in the stem bark of F. sycomorus L. Methodology: The stem bark of F. sycomorus L was collected, air dried, and successively extracted with petroleum ether, ethyl acetate, and methanol, and the extracts were screened for their chemical constituents and studied there in vitro antibacterial and antioxidant activities. The antimicrobial activity of the plant extracts was assayed using the agar plate disc diffusion and also antioxidant activity of plant extracts was assayed using DPPH assay. The essential oils were extracted using the hydro distillation method, and their chemical compositions were characterized using GC-MS. Result: The qualitative phytochemical analysis results revealed the presence of terpenoids, steroids, phenol, alkaloids, saponins, flavonoids, and coumarins. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were also determined. As a result, the TPC of methanol extract was found to be 45.61 mg GAE/g, and the TFC was 6.006 mg QE/g while the TPC of ethyl acetate extract was found to be 39.34 mg GAE/g and the TFC was 3.021 mg QE/g. The antibacterial activity study of methanol extract was better than that of ethyl acetate. The zone of inhibition for methanol extract was varied from 0-11 mm whereas for ethyl acetate extract it was varied from 0-9 mm. the maximum zone of inhibition of the extract (11 and 9 mm) was recorded at 40 mg/mL. Antioxidant activity of ethyl acetate and methanol crude extracts was also evaluated by DPPH assay, and the results showed inhibitory concentration (IC 50) values of methanol extract was 0.10065 and 0.13354 mg/mL ethyl acetate extract. GC-MS analysis of essential oils gave 28 compounds. Terpenoids, phenols, and esters were the major components of essential oil. Conclusion, the methanol extract had become higher in all parameters such as antioxidant activities, total phenolic content, total flavonoid content, and antibacterial activity than the ethyl acetate extracts. This result partly explains why solvent polarity has a significant effect on the extracted materials of a given sample. Key words: Total phenolic content, Total flavonoid content, Antibacterial, Phytochemical analysis, and Antioxidant.	en_US
dc.language.iso	en_US	en_US
dc.subject	Chemistry	en_US
dc.title	Phytochemical Investigation, In Vitro Anti-Bacterial and Anti-Oxidant Activity and Gc-Ms Profile Of Essential Oils of Stem Bark Extract of Ficus Sycomorus L. (Bamba)	en_US
dc.type	Thesis	en_US