PHYTOCHEMICAL INVESTIGATION AND DETERMINATION OF  BIOLOGICAL AND ANTIOXIDANT ACTIVITIES OF THE LEAF EXTRACT  OF WITHANIA SOMNIFERA (GIZAWA

TESEMA, TARAKEGN

dc.contributor.author	TESEMA, TARAKEGN
dc.date.accessioned	2021-11-10T06:49:16Z
dc.date.available	2021-11-10T06:49:16Z
dc.date.issued	2021-11-10
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/12902
dc.description.abstract	Most plant extracts are considered to have phytochemicals that are responsible for the treatment of various ailments and hence termed as ‘medicinal plants’. Withania somnifera (gizawa in Amharic) was known for the treatmentof anxiety, cognitive and neurological disorders, and inflammation. The aim of this study is to extract phytochemicals from leaf part of Withania somnifera and study antioxidant and antibacterial activity of the crude plant extracts. Qualitative analysis of ethanol extract of leaf of Withania somnifera revealed the presence of phenolics, flavonoids, tannins, terpenoids, saponins, phytosterols and steroids. Phenolics, saponins, terpenoids, alkaloids and flavonoids were present in ethyl acetate extract while petroleum ether extract confirmed the presence of phenolics, tannins, flavonoids and terpenoids. The ethanol, ethyl acetate and petroleum ether extractswerefound to have total flavonoid content of 63.57 mgQE/g, 37.26 mgQE/g and 25.98 mgQE/g respectively. All extracts showed antioxidant capacity using DPPH free radical scavenging activity and ferric reducing antioxidant power (FRAP) assay. IC50 vlaues of ethanol, ethyl acetate and petroleum ether extracts were 51.59 µg/mL,59.8 µg/mL and 111.3 µg/mL respectively. Antibacteral activity test was done by using agar disk diffusion method against four bacterial strains: two gram negative bacteria (E. coli and K. pneumoneae) and two gram posative bacteria(S. aureus andS. pyogens). Petroleum ether and ethanol extracts showed good antibacterial activity against S.aureus. Ethyl acetate extract possess a highest zone of inhibition against K. pneumoneae.	en_US
dc.language.iso	en	en_US
dc.subject	Chemistry	en_US
dc.title	PHYTOCHEMICAL INVESTIGATION AND DETERMINATION OF BIOLOGICAL AND ANTIOXIDANT ACTIVITIES OF THE LEAF EXTRACT OF WITHANIA SOMNIFERA (GIZAWA	en_US
dc.type	Thesis	en_US