PHYTOCHEMICAL ANALYSIS, ANTIBACTERIALACTIVITY AND ANTIOXIDANT  ACTIVITY STUDIES ON THE CRUDE EXTRACT OF STEM BARK OF CLAUSENA  ANISATA

NAMARA, GARAMU

dc.contributor.author	NAMARA, GARAMU
dc.date.accessioned	2021-11-10T06:33:20Z
dc.date.available	2021-11-10T06:33:20Z
dc.date.issued	2021-11-10
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/12900
dc.description.abstract	This study is aimed to evaluate the phytochemical constituents, antibacterial and antioxidant activities of the crude extracts of stem bark of C. anisata. The air-dried stem bark was powdered and extracted successively with petroleum ether, ethyl acetate and methanol. The semi-solid matter was separated by filtration and then the filtrates were concentrated using a rotary evaporator. The extract was subjected to a preliminary phytochemical screening test using standard procedures and the result showed the presence of alkaloid, tannins, terpenoids, flavonoids, phenolics, glycosides, steroids and anthraquinones and absence of saponin. Antibacterial activity of the crude extract was determined by using the Agar disc diffusion method against four bacterial strains (two gram positive): Staphylococcus aureus, Streptococcus pyogenes and two gram negative: Escherichia coli, Klebsiella Pneumonie. The methanol extract of the stem bark of C. anisata showed a significant inhibition zone on all of the selected bacteria. While ethyl acetate extract had medium inhibition on all of the selected bacteria. Each crude extract was screened for antioxidant activity by the DPPH assay method and FRAP assay method. Aluminum chloride colorimetric method was used to determine the flavonoid contents in these extracts. The results showed that the methanol extract of the stem bark exhibited the highest antioxidant activity of 77.77 % in DPPH with an IC 50% value of 41.3 ppm. In FRAP assay 69.11% with an IC 50% value of 51.498 ppm, which appeared to contain more flavonoid contents 127.5 mg QE /g of sample. The results of this study showed that the stem bark of C. anisata constitute phytochemicals that possess antibacterial and antioxidant activities and can serve as a potential source in search of plant-based antibiotics and natural antioxidants.	en_US
dc.language.iso	en	en_US
dc.subject	Chemistry	en_US
dc.title	PHYTOCHEMICAL ANALYSIS, ANTIBACTERIALACTIVITY AND ANTIOXIDANT ACTIVITY STUDIES ON THE CRUDE EXTRACT OF STEM BARK OF CLAUSENA ANISATA	en_US
dc.type	Thesis	en_US