Phytochemical analysis, antioxidant and antibacterial activities of leaf extracts  of calpurnia aurea

Hunde, Debele

dc.contributor.author	Hunde, Debele
dc.date.accessioned	2021-11-10T06:27:03Z
dc.date.available	2021-11-10T06:27:03Z
dc.date.issued	2021-11-10
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/12899
dc.description.abstract	The leaf of Calpurniaaurea,one of the most popular herbal products in tropical countries, is used in traditional medicine for treatment of several diseases. The leaf part this plant was extracted by successive extraction. The present study was conducted to investigate the anti-oxidative and antibacterial activities of different solvent extracts of the leaf of C.aura. The results indicated that all extracts have reducing power of DPPH radical scavenging abilities. Methanol and ethyl acetate extracts have the highest total reducing power and petroleum ether extract showed the lowest reducing power. Qualitative phytochemical screening of the extracts indicated the presence of glycoside, steroids, terpenoids,saponins and tannins with different concentration. Quantitative analysis of the leaves extracts of C.aurea for flavonoid was performed. The total flavonoid content varied from 41to134.58 mg/g of dry weight of extracts that expressed as Quercetin equivalents. The antioxidant activities of methanol, ethyl acetate and petroleum ether leaf extracts of Calpurnia aurea were screened using 1.1-diphenyl-2-picryhydrazyl and ferric reducing power assay.IC50 values were found to be 53μg/mL, 82.541μg/mL and 172.103 μg/mL in methanol, ethyl acetate and petroleum ether leaf extracts respectively for their antioxidant activity by DPPH assay; the lower IC50 value which showed the radical scavenge activity to be effective in this solvent extract. In the same condition methanol, ethyl acetate and petroleum ether extract showed high antioxidant activity and oxygen free radicals scavenging abilities which were found almost equivalent to the ascorbic acid standard antioxidant activity. The antibacterial activity of plant extract of C.aurea was performed against four bacteria species S. aureus, E coli, S. pyogens, K. pneumonia, at concentrations of 1000 mg/L. The result revealed that the methanol and ethyl acetate extracts showed a better activity on all disease causing pathogens bacteria than petroleum extract	en_US
dc.language.iso	en	en_US
dc.subject	Chemistry	en_US
dc.title	Phytochemical analysis, antioxidant and antibacterial activities of leaf extracts of calpurnia aurea	en_US
dc.type	Thesis	en_US