POLY (DIPHENYL AMINE-4- SULFONIC ACID) MODIFIED GLASSY CARBON  ELECTRODE FOR SELECTIVE VOLTAMETRIC DETERMINATION OF GALLIC  ACID IN HONEY SAMPLES FROM GAZO WEREDA OF NORTH WOLLO ZONE,  AMHARA REGION

MISGAN, BEYENE

dc.contributor.author	MISGAN, BEYENE
dc.date.accessioned	2021-10-28T11:07:46Z
dc.date.available	2021-10-28T11:07:46Z
dc.date.issued	2021-10-28
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/12887
dc.description.abstract	This work describes the development of an electrochemical method based on a novel poly (diphenylamine-4-sulfonic acid) modified glassy carbon electrode (poly (DPASA/GCE) for determination of GA in honey samples. The deposition of the polymer film (poly(DPASA) on surface of GCE was characterized by cyclic voltammetry using a redox probe (Fe (CN) 6 3-/4- ) complemented with electrochemical impedance spectroscopy. In comparison to the unmodified glassy carbon electrode, poly (DPASA) modified GCE revealed a well-defined, sharp oxidative peak with much enhanced peak current and reduced over-potential for gallic acid. Improved correlation coefficient for the dependence of peak current on the scan rate (R2=0.999192) than on the square root of scan rate (R2=0.97471) indicated that the oxidation of GA at poly (PDASA)/GCE was mainly adsorption-controlled. Under optimized condition the current response of poly (DPASA)/GCE showed linear dependence on the concentration of gallic acid in the range of 1.0–300.0 µM with a limit of detection and limit of quantification of 0.00446 and 0.0148 µM, respectively. Spike recovery results in the range 96.97-98.33% and interference recovery in the range 98.28- 102.08% in the presence of 50-200% of AA and UA, validated the developed method for determination of gallic acid in honey sample. The Gallic acid content of the honey samples collected from the three sampling sites was 21.0 µM (01kebele), 13.07 µM (09kebele) and 2.66 µM (014kebele). The current method had a better performance for determination of GA in honey sample as compared with recently reported voltammetric methods, due to its requirement of simple electrode modification step, extremely low detection limit, provides the least limit of detection, and reasonably wider linear dynamic range. It was indicating the capable applications of the method for determination of GA in real samples including honey and food samples.	en_US
dc.language.iso	en	en_US
dc.subject	Chemistry	en_US
dc.title	POLY (DIPHENYL AMINE-4- SULFONIC ACID) MODIFIED GLASSY CARBON ELECTRODE FOR SELECTIVE VOLTAMETRIC DETERMINATION OF GALLIC ACID IN HONEY SAMPLES FROM GAZO WEREDA OF NORTH WOLLO ZONE, AMHARA REGION	en_US
dc.type	Thesis	en_US