ANTIMICROBIAL ACTIVITY OF ACTINOMYCTES AND  DRUG RESISTANCE PROFILE OF COMMON BACTERIA  ISOLATED FROM MUNICIPAL WASTE DUMP SITE  INBAHIR DAR CITY,  ETHIOPIA

AYALEW, FIKREMARIAM

dc.contributor.author	AYALEW, FIKREMARIAM
dc.date.accessioned	2021-07-26T13:07:58Z
dc.date.available	2021-07-26T13:07:58Z
dc.date.issued	2021-07-26
dc.identifier.uri	http://ir.bdu.edu.et/handle/123456789/12257
dc.description.abstract	The emergence of multidrug resistance (MDR) at an alarming rate among the microbial population has been a public health concern. Globally, finding new antimicrobials that can kill multidrug resistant pathogens is thus a hot research area. Bacteria, specifically actinomycetes, are the most prolific producers of antibiotics. Municipal waste has been a reservoir for multidrug resistance pathogens due to the unwise disposal of leftover drugs from households. This study aimed to identify multidrug resistance and antimicrobial-producing bacteria in a municipal solid waste soil from dump site in Bahir Dar, Ethiopia. Thirty soil samples were randomly collected from the municipal waste dump site in three rounds from February up to June, and analyzed by using SPSS soffit ware version 26. Actinomycetes and other bacteria were isolated from these samples using the spread plate method on starch casein, and nutrient agar medium respectively. The isolates were screened for antimicrobial activity against at least one bacterial test strain by perpendicular streaking. Isolates showing antimicrobial activity were further screened for better potential using an agar well and disk diffusion method from an ethyl acetate crude extract of a bacterial culture. Isolates showing both antimicrobial activity and drug resistance were identified using cultural methods. Thirty nine bacterial isolates were subjected to different antibiotic disks to test antibiotic susceptibility tests by the standard Kirby-Bauer's disc diffusion method. Regarding the drug resistance profile, of a total of Thirty nine bacterial isolates, 38.4% were resistant; 21.74% of isolates were intermediate and 39.86% of bacterial isolates were susceptible to selected antibiotic disks and generally 46.15% of the bacterial isolates were multidrug resistance, from this the most MDR isolates were S. aureus & E.coli. According to this study, for chloramphenicol and ciprofloxacin most isolates were susceptible, but for amoxicillin and Nalidixic acid most bacterial isolates were resistance. S.aureus and E.coli were shows the most resistant from the other isolate. From a total of 70 actinomycetes, 20 (28.57%) showed antimicrobial activities with the inhibition zone ranging from 0.0±0.0 up to 26.0±1.0 mm on average, in both agar well and disk diffusion methods. Twenty effective actinomycetes were selected for MIC & MBC, and they have the value of MIC and MBC between 250µg/ml to 500250µg/ml. Based on antibacterial activities, two isolates were selected for the MIC and MBC tests against E.coli and S. aureus. From the present study it could be conclude that sebatamite municipal waste site was good source of antibiotic producing actinomycetes and it also contain most abundance of multidrug resistance. Further work needs to identify all antibiotic producing and drug resistance microorganism up to species level by using molecular characterization. Key words: Antimicrobials, Actinomycetes, bacteria, drug resistance, waste dump sites	en_US
dc.language.iso	en	en_US
dc.subject	Biology	en_US
dc.title	ANTIMICROBIAL ACTIVITY OF ACTINOMYCTES AND DRUG RESISTANCE PROFILE OF COMMON BACTERIA ISOLATED FROM MUNICIPAL WASTE DUMP SITE INBAHIR DAR CITY, ETHIOPIA	en_US
dc.type	Thesis	en_US