The improper use of antibiotics has led to an increase in drug-resistant bacteria against known antibiotics. To overcome the alarming problem of microbial resistance to antibiotics, the discovery of novel active metal complexes derived from biologically active heterocyclic aromatic ligands for drug development against new targets is a matter of urgency. Based on this, in the present study two new Iron (III) complexes [Fe(phen)2(en)(H2O)]Cl3 mononuclear and [Fe2(phen)4(en)(H2O)2]Cl6 binuclear were synthesized from FeCl3, 1,10-phenanthroline monohydrate, and ethylenediamine by drop wise addition of ligand solution to the salt. The structures of the synthesized complexes were characterized using various Physico-chemical (Chloride test, molar conductance, solubility test, and melting point) and spectroscopic techniques (FTIR, UV-Vis, ICP-OES analysis). The change in color, solubility, melting point, peak position in the FT-IR and UV-Vis from the ligand indicate the formation of metal complexes. Based on the analytical and spectroscopic data Octahedral geometry were presumed to be achieved. The synthesized metal complexes have been evaluated for their in-vitro antibacterial activity against some Gram-positive (Staphylococcus aureus, Streptococcus pyogenes) and Gram-negative (Escherichia coli and Klebsiella Pneumonia) bacteria by Disc diffusion method. The comparative antibacterial study was also conducted using commercial drug Gentamycin as reference material. The synthesized complexes showed even higher antibacterial activity on (S. aurous and K. Pneumonia) and comparable antibacterial activity on (S.pyogenes and E.Coli) compared to the standard antibiotic drug Gentamycin. Thus, the synthesized complexes may be considered as potential antibacterial agents after passing cytotoxicity testing