This research focuses onthe evaluation ofphysicochemical and bacteriological drinking water quality variation in Injibara town spatially and temporally.These results were compared with WHO and Ethiopia drinking water quality standards. Also, the assessment of residual chlorine concentration was done in the distribution network at two hrs interval four times for one day monthly. Water samples were collected in triplicates from 18 selected sampling points from March 2019 to August 2019. A total of 300 water samples were collected and results showed that most of the samples were within the range of Ethiopia and WHO standards. However, iron from both borehole sources, TDS from Borehole (BH2) in the dry and wet season, and turbidity from spring in the wet season were above the permissible limit. All sources of water including on spot sources were positive for total coliform in both seasons. In the distribution system all samples were within permissible limit except TDS in distribution sample point DS6, total coliform from (Ds6) and (Ds9), turbidity from DS4 and DS5 as well as residual chlorine recorded in the majority of the water samples were less than WHO standard.Based on the results, it was foundthat water quality deterioration was found both at the sources and in the supply networks in both seasons. Hence, construction and management of toilet facilities, proper drainage, proper sewage disposal systems, and additional treatments is necessary before using for direct consumption at one spot springs and sufficient disinfection of water with chlorine (chlorine dosing) for main sources is needed.