ETHYLENE DIAMINE TETRA ACETIC ACID DISODIUM SALT MODIFIED CARBON PASTE ELECTRODE FOR SQUARE WAVE  VOLTAMMETRIC DETERMINATION OF THEOPHYLLINE IN PHARMACEUTICAL TABLET FORMULATION

MOGES, AMSALU

dc.contributor.author	MOGES, AMSALU
dc.date.accessioned	2020-11-06T10:59:52Z
dc.date.available	2020-11-06T10:59:52Z
dc.date.issued	2020-11-06
dc.identifier.uri	http://hdl.handle.net/123456789/11580
dc.description.abstract	Theophylline is one of the important natural alkaloids effective in the treatment of respiratory disease. While the level of theophylline in adult plasma below 5-20 µg/mL is none therapeutic, its amount above the level is known to be toxic necessitating continuous monitoring of its content both in human fluids and pharmaceutical formulations including tablet. In this study, an electrochemical method using ethylenediaminetetraacetic acid disodium salt modified carbon paste electrode for determination of theophylline in the tablet formulation is presented. The modified electrode was characterized using cyclic voltammetry; Fourier transforms infrared and electrochemical impedance spectroscopy. Theophylline showed irreversible oxidation with peak current enhancement at 10% ethylenediaminetetraacetic acid disodium salt modified carbon paste electrode than at the unmodified carbon paste electrode. Investigation of the dependence of anodic peak current and peak potential at the modified electrode on scan rate showed that the rate of the irreversible oxidation of theophylline was predominantly diffusion mass transport controlled. Under the optimized conditions, square wave oxidative peak current response of the modified electrode for theophylline in pH 7.0 PBS showed a linear dependence on the concentration in the range 10-200 µM with a determination coefficient (R2), the limit of detection, and limit of quantification of 0.99782, 0.0257 µM, and 0.0857 µM, respectively. Recovery of 98.24% for spiked standard theophylline in tablet sample and 95.7-100% in the presence of caffeine, uric acid, and ascorbic acid as potential interferents validated the applicability of the developed method for determination of theophylline content of tablet formulation. The theophylline level of a tablet formulation labeled 120 mg/tablet determined using the developed method was 122.22 mg/tablet. The detected level with 1.85% deviation from the expected level confirmed the accuracy of the developed method which farther validated our method as a comparable potential candidate with other methods for its application.	en_US
dc.language.iso	en_US	en_US
dc.subject	chemistry	en_US
dc.title	ETHYLENE DIAMINE TETRA ACETIC ACID DISODIUM SALT MODIFIED CARBON PASTE ELECTRODE FOR SQUARE WAVE VOLTAMMETRIC DETERMINATION OF THEOPHYLLINE IN PHARMACEUTICAL TABLET FORMULATION	en_US
dc.type	Thesis	en_US