STUDIES ON ANTI-OXIDATIVE AND ANTIBACTERIAL ACTIVITIES OF THE LEAVES EXTRACT OF CLEMATIS HIRSUTA (Nech yeazo hareg)

AZEMERAW, MOLLA

dc.contributor.author	AZEMERAW, MOLLA
dc.date.accessioned	2020-10-13T07:48:57Z
dc.date.available	2020-10-13T07:48:57Z
dc.date.issued	2020-10-12
dc.identifier.uri	http://hdl.handle.net/123456789/11350
dc.description.abstract	ABSTRACT Clematis hirsuta plant is one of the commonly used traditional medicinal plants in Ethiopia for treating wounds, headache, and cough. The current study intended to investigate the antibacterial and anti-oxidative activities of the crude extract of the leaves obtained using petroleum ether, chloroform, and ethanol. Antioxidant and antibacterial activities of these three solid fractions were also investigated. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and ferric reducing antioxidant power (FRAP) assays. The antibacterial activity was examined using the Agar disk diffusion method for all the crude extracts Folin Ciocalteu method was used to determine the total phenolic and aluminum chloride colorimetric method was used to determine the flavonoid contents in these extracts. The results showed that the ethanol extract of the leaves exhibited the highest antioxidant activity of 82.86 % in DPPH with an IC 50% value of 43.15 ppm. In FRAP assay 36.923 % with an IC 50% value of 118.30 ppm, which appeared to contain more phenolic and flavonoid contents (163.62 mg GA/g, and 161.00mgCE/g of sample). The chloroform extract exhibited the highest antibacterial activity against three bacterial strains (S. aureus, S. pyogenes, and E. coli). This study provides evidence that the leaves of the Clematis hirsuta could be a good source of natural antioxidants, and antibacterial agents. Keywords: Clematis hirsuta, antioxidant activity, antimicrobial activity, total phenolic content and total flavonoid contents, isolation, UV Vis, FTIR spectroscopy	en_US
dc.language.iso	en	en_US
dc.subject	Chemistry	en_US
dc.title	STUDIES ON ANTI-OXIDATIVE AND ANTIBACTERIAL ACTIVITIES OF THE LEAVES EXTRACT OF CLEMATIS HIRSUTA (Nech yeazo hareg)	en_US
dc.type	Thesis	en_US