Despite bicycling is fun, healthy, economical, efficient, and considered as one of a sustainable mode of transport, in Bahir Dar, the level of use of bicycle decreases time to time due to the existence of different barriers to bicycling. The objectives of the research were to identify the barriers and motivations to bicycling; to differenciate the degree of influence of barriers as it differes on socio-demographic characterstics of residents of Bahir Dar City; and to evaluate the suitability of selected road segments for riding a bicycle. To accomplish the research objectives, questionnaires were developed after reviewing different researches to identify barriers and motivations; to examine the interaction between vehicle drivers and bicyclists; and to identify which road segments are relatively suitable to ride a bicycle. Factor analysis and Mean score analysis by IBM SPSS, BLOS analysis by HCM 2010 were done to identify the top barriers; motivations to bicycling; the interaction between drivers and cyclists; and the suitability of selected road segments for riding a bicycle. About 308 respondents including currently-cyclists and cyclists in the past were participated in the rating of variables. The result revealed that Infrastructure and road traffic; safety and security; and on-street activities related barriers were identified as the top three barriers to bicycling. The interaction between cyclists and vehicle drivers especially Bajaj drivers were also identified as the major barriers. It was identified that Provision and improvements of cycling infrastructure facilities such as segregated bicycle lane and bicycle parking can be the top motivation to cyclists. Cyclists have been using bicycle as transport mode due to advanteges cycling such as environment friendliness of bicycle; and healthiness of bicycle. Most of the road links on selected road segments have a Bicycle Level of Service (BLOS) of “D” and “C” and rated as unsuitable which implies that bicyclists feel unsafe and uncomfortable to ride a bicycle. It is recommended that the City Municipality and Road and Transport Sectors develop policies and implement soft and hard measures for the encouragements of bicyclists. Treatments such as providing speed limits of 30km/hr for vehicle; removing the on-street parking; and provision of bicycle lane identified as the top powerfull measures to improve significantly the Bicycle Level of Services.