Plumbago zeylanica (Amera) is one of the most versatile medicinal plants having a wide spectrum of biological activities due to the presence of large number of bioactive compounds. The present study is conducted to evaluate phytochemical test, antimicrobial activity, antioxidant efficiency, total phenolic and flavonoid content using methanol, ethyl acetate and petroleum ether extracts. Qualitative phytochemical screenings were also conducted using methanol, ethyl acetate and petroleum ether extracts, which revealed the presence of cardiac glycosides, flavonoids, phenolic compounds, tannins, sterols, quinone, anthraquinone, terpenoids, cholesterol, protein and alkaloids The antibacterial activity of leaf extract of Plumbago zeylanica was performed against four bacterials species S. aureus, E coli, S. pyrogens, K. pneumonia, at concentrations of 500 mg/mL,400 mg/mL,300 mg/mL and 200 mg/mL and 100 mg/mL . The result revealed that the methanol and ethyl acetate extracts showed a better activity against all disease causing pathogens bacterial while, at higher concentration petroleum ether extracts were active against microorganisms.The total phenolic content of methanol, ethyl acetate and petroleum ether extract was 82.738 mgGAE/g, 69.784 mgGAE/g and 51.630 mgGAE/g dry samples respectively. The total flavonoid content of methanol, ethyl acetate and petroleum ether extract was 69.566 mgQE/g, 66.966 mgQE/g and 59.133 mgQE/g dry samples respectively. The antioxidant activities of methanol, ethyl acetate and petroleum ether leaf extracts of Plumbago zeylanica were screened using 2.2-diphenyl-2picryhydrazyl and ferric reducing power assay. IC values were found to be 53.143 μg/mL , 82.541 μg/mL and 172.103 μg/mL in methanol, ethyl acetate and petroleum ether leaf extracts respectively for their antioxidant activity by DPPH assay; the lower IC 50 value which showed the radical scavenge activity to be effective in this solvent extract. In the same condition methanol, ethyl acetate and petroleum ether extract showed high antioxidant activity and oxygen free radicals scavenging abilities which were found almost equivalent to the ascorbic acid standard antioxidant activity. Key words: - Plumbago zeylanica, phytochemical analyses, Antioxidant activities, total phenolic content, total flavonoid content and antimicrobial activity 50