Abstract The study was conducted from October-2010 to April-2011 in and around Bahir Dar (Ethiopia) to identify the major gastrointestinal helminths of equines (donkeys and mules), to determine their prevalence rates and find associations between measurable parameters and parasites burden. A total of 384 faecal samples (212 donkeys and 172 mules) were collected randomly for qualitative and quantitative faecal analysis. The overall prevalence of different parasites was found to be 88.21% in donkeys and 77.91 % in mules. In the study area, 5.88% of donkeys and 15.67% of mules harbored only one type of parasite (single infection), whereas 94.1 % of donkeys and 84.33% of mules harbored two or more types of parasites (mixed infection). The parasites encountered in both donkeys and mules in the study period were Strongyles (65.09% and 66.28%), T strongylus axei (42.45% and 31.97%), T dontophorus (36.32% and 33.72%), T nema (34.91 % and 37.79%), P equorum (13.68% and 10.46%), D arnfieldi p2. I 7% and 8.14%), A. cephala (23.12% and 16.86%) and Fasciola ( 17.92% and 13.95%), respectively. From the positive donkeys and mules, 46.82% of donkeys were severely infected, 86.31 % moderately and 69.4 7% mildly; whereas 39.42% of mules were infected severely, 93.43% moderately and 80.29% mildly. Based on sex, the prevalence rates of all identified parasites were statistically significant (p<0.05) in donkeys. In contrast, all studied parasites were not statistically significant in mules (p>0.05). The prevalence ofstrongyles, T strongylus, T dontophorus. T Nema, and P equorom was statstically significant (p<0.05) between age groups of donkeys, but the prevalence of A. cephala and Fasciola was not statistically significant (p>0.05). In mules, the prevalence of strongyles, T dontophorus and T nema was statistically significant (p<0.05), but the prevalence of T strongylus, P equorum, D. arnfieldi, A. cephala, and Fasciola was not statistically significant (p>0.05) between age groups of mules. The body condition score was negatively correlated (r=-0.664 for donkeys and r=-0.637 for mules, respectively) with the the total EPG counts. Parasitism and other health problems were identified affecting the health and welfare of equines. Government or other development agencies should include donkeys and mules in their priority lists of research and develop sustainable prevention and control methods that would prevent the high transmission